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Tear Biomarkers in Veterinary Species: An Updated Review With Special Focus on Dogs and Cats

An updated review examines tears as a source of biomarkers in veterinary species, with emphasis on dogs and cats. The paper discusses collection, analytes, ocular and systemic applications, and technical limitations that affect whether tear testing can move from research into routine practice.

Primary source: PubMed / The Veterinary Journal
Published: 2026-07-16
Reviewed and summarized by the AlmostAVet Editorial AI
Jul 16 2026
At a Glance

What This Means for Different Readers

Three quick summaries of the same article, tailored for different readers.

🏠
Pet Owner

Could a tear sample reveal more than an eye problem?

Tear collection may eventually offer a low-stress way to monitor certain conditions, but results can change with irritation, reflex tearing, sample method, and storage. A new test must be validated before a number can reliably guide treatment. Owners should be cautious about commercial claims that move faster than the evidence.

The review summarizes current veterinary research and limitations.
🧪
Vet Tech

Tear diagnostics begin with preanalytic control

Technicians may eventually play a major role in standardized tear sampling, but research protocols should not be improvised into clinical interpretation. Record which eye was sampled, topical medications, sedation, ocular disease, collection duration, environmental conditions, and processing time. Reproducibility matters before a biomarker becomes useful.

The review details candidate analytes and collection challenges.
🎓
Pre-Vet

Tears offer a minimally invasive matrix with difficult validation problems

A biomarker requires analytic validity, biologic plausibility, reference intervals, repeatability, and evidence that it improves a clinical decision. Tear composition is influenced by lacrimal secretion, ocular surface disease, reflex response, and collection technique. The field illustrates the long path from detectable molecule to clinically actionable test.

The PubMed record links to the complete review.
Key Takeaway
A sample that is easy to collect is not automatically easy to interpret. Collection method, tear volume, reflex tearing, storage, assay validation, and species differences all influence the result.